In order to facilitate the accessibility of the proposed predictive model by the scientific community, we have developed a user-friendly webserver by the same name of NEPTUNE, which is made freely available online at http://pmlabstack.pythonanywhere.com/NEPTUNE. Herein, we provide step-by-step guidelines on how to use the NEPTUNE webserver in order to obtain the prediction results.

Firstly, opening the webserver at http://pmlabstack.pythonanywhere.com/NEPTUNE. Secondly, the user enters the query sequence into the text box. Thirdly, the user clicks on the “Submit” button in order to start the prediction process. Typically, in this step, it takes a few seconds for the server to process the task. Finally, after finishing the prediction process, the results are displayed as shown on the right-hand side of the webserver. The user can see examples of FASTA-formatted sequences by clicking on the “example file” button.