In order to facilitate the high-throughput identification of TTCA and accessibility of the proposed predictive model by the scientific community, we have developed a user-friendly webserver named StackTTCA.

Herein, we provide step-by-step guidelines on how to use the StackTTCA webserver in order to obtain the prediction results. Firstly, opening the webserver at http://pmlabstack.pythonanywhere.com/StackTTCA. Secondly, the user enters the query FASTA into the text box. Thirdly, the user clicks on the “Submit” button in order to start the prediction process. Typically, in this step, it takes a few seconds for the server to process the task. Finally, after finishing the prediction process, the results are displayed as shown on the right-hand side of the webserver. The example file provided can help the user to see how to use the FASTA notation for obtaining the prediction. Users can access the file by clicking on the “example file” button.